Targetable IDH1 mutation identified in a rare case of pancreatic serous cystadenocarcinoma but not a series of serous cystadenomas

Abstract

INTRODUCTION

Pancreatic cystic neoplasms are divided into four categories: serous cystic neoplasms (SCN), mucinous cystic neoplasms (MCN), intraductal papillary mucinous neoplasms (IPMN) and solid pseudopapillary neoplasms (SPN) [1]. SCN of the pancreas is a well-recognized, largely benign class of pancreatic cystic neoplasms [1]. Nevertheless, serous cystadenoma (SCA) may have the potential to progress to serous cystadenocarcinoma (SCAC), a rare diagnosis first described in 1989 [2]. To date, 35 malignant pancreatic SCN cases (15 with distant metastases) have been reported worldwide [3]. A recent systematic review showed overall favorable outcome for SCAC with 77% (17/22) alive at a median follow-up of 2 years [4]. Definitive diagnosis of SCAC compared with SCA is challenging given similar clinical, radiographic and even pathologic findings with SCA, with a key distinguishing feature being distant metastasis [4–6].

In addition to the analysis of cyst fluid CEA and amylase, molecular markers have been applied to categorize pancreatic cystic neoplasms. Alterations in VHL and GNAS appear unique to SCA and IPMN, respectively, while mutations in KRAS and RNF43 are associated with both IPMNs and MCN [1], and alterations in CTNNB1 may be seen in SPN and IPMN [1]. Current guidelines do not support mutational analysis for SCN diagnosis but recommend such testing if the diagnosis of IPMN or MCN is unclear [7]. To date, genomic analysis of SCAC has not been reported. It is unknown whether SCA is a precursor lesion to SCAC, and factors associated with progression from SCA to SCAC are unknown. One potential molecular marker for SCAC is the VHL gene mutation, which has been reported in somatic SCA samples [1, 8]. VHL is an autosomal dominant tumor suppressor gene, and mutations are associated with von Hippel–Lindau (VHL) syndrome, a complex syndrome that includes cysts in multiple organs, such as the pancreas and the kidney, and other neoplasms [9]. Here, we present a case of pancreatic SCAC, with retrospective comparison of clinical and molecular data to five SCA using an institutional review board-approved protocol.

CASES REPORT/SERIES

A 74-year-old woman with a history of hypertension, hypercholesterolemia and gastroesophageal reflux presented with dyspnea and uncontrolled hypertension. Her past surgical history included wrist surgery and a cholecystectomy. Family history was notable for leukemia, lung, extrahepatic bile duct and colon cancer. Other symptoms included early satiety and increased gastroesophageal reflux. Pulmonary embolism was initially suspected, but computed tomography instead showed a 10 cm, multi-cystic, partially calcified mass in the tail of pancreas. Percutaneous biopsy was performed revealing an SCN. Magnetic resonance (MR) imaging revealed an 11.5-cm lesion in the pancreatic body and tail. On T₂-weighted images, a microcystic mass with a bosselated surface and a central dark scar (Fig. 1A) was seen. Malignancy was suspected on the basis of invasion into the splenic parenchyma and a cystic lesion adjacent to the liver with similar features (Fig. 1B).

Figure 1

Imaging and pathology of serous cystadenocarcinoma of the pancreas. (A) T₂-weighted MR image showing microcysic lesion with invasion into spleen (red arrow) and central dark scar (arrowhead) with (B) peritoneal lesion at inferior tip of the liver (blue arrow). (C) Gross specimen photograph demonstrating the large pancreatic mass (white arrow) with invasion into the spleen (S) and central necrosis (scale bar, 1 cm). (D) Photomicrograph demonstrating invasion of the splenic parenchyma (superior, H&E, 20×).

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The patient underwent a distal subtotal pancreatectomy/splenectomy and resection of the cystic lesion adjacent to the liver in 2018. Surgical pathology revealed an 11.5 × 10.0 × 9.0 cm mass that largely replaced the pancreatic body and tail. This mass had a pinkish, glistening, solid and cystic cut surface with central chalky white necrosis (Fig. 1C). This lesion was grossly adherent to the splenic hilum. The surgical margins were widely uninvolved by the tumor. Microscopically, the tumor had the classic morphology of a serous cystic neoplasm of the pancreas with cysts lined by cuboidal to flattened cells with bland, central, round nuclei and abundant clear cytoplasm. PAS stains demonstrated abundant glycogen. There were large areas of coagulative necrosis, and the tumor focally invaded the splenic parenchyma grossly (Fig. 1C) and microscopically (Fig. 1D). Mitotic figures were not identified. The separately submitted perihepatic nodule showed identical morphology, consistent with a distant metastasis. Altogether, these findings were diagnostic of a serous cystadenocarcinoma. An outside consultation was obtained from the Johns Hopkins Department of Pathology which confirmed the diagnosis.

Post-operatively, the patient had a prolonged hospital course and multiple readmissions related to a post-operative pancreatic fistula. She was found to have pancreas divisum, and her management required an interventional radiology drain, an endoscopic cyst-gastrostomy and an endoscopic retrograde cholangio-pancreatography with placement of a stent in her dorsal and ventral pancreatic ducts; 32 months post-operatively, she was clinically well and without evidence of disease.

The SCAC case was compared with five archival cases of SCA resected from 2016 to 2019, identified from an institutional pathology database. Among these six total patients with SCN of the pancreas, the majority were female (Table 1). None carried a clinical diagnosis or family a history of VHL syndrome. Compared with the 5 SCA patients, the SCAC patient was slightly older (74 vs median 70 years old) at surgery. Compared with the resected SCAs, the SCAC was larger (11.5 vs median 3.8 cm). The SCAC and 3 SCA were resected for symptoms, while the other 2 SCA were resected for indeterminate imaging findings. The SCAC spanned the body and tail of pancreas and the locations of the 5 SCAs were as follows: 2 tail, 1 neck, 1 body and 1 head. Distant metastasis was only observed in the SCAC as a small peritoneal nodule adjacent to the liver. Benign splenic simple cysts and multiple benign multiple peritoneal mesothelial inclusion cysts were noted in two SCAs, respectively. The majority of patients underwent distal pancreatectomy/splenectomy (SCAC and 4 out of 5 SCAs). All patients were alive and without recurrence at last follow-up with a median follow-up period of 12 months after surgery.

Somatic Whole Exome Sequencing (WES) was performed on the resected specimens. No mutations were seen in KRAS, GNAS, RNF43 or CTNNB1 as previously described in non-serous cystic pancreatic lesions [1, 8]. VHL mutations (N78S, S68S?, L63 and S80R) were found in 4 SCAs and another (PYPTL97) in the SCAC (Table 1). One of these mutations in an SCA was labeled clinically pathogenic by ClinVar [10], a database annotating observed clinical phenotype with genomic variants, while another was designated as pathogenic using Functional Analysis through Hidden Markov Models (FATHMM), an algorithm for predicting pathogenicity of a mutation [11]. The remaining VHL mutations were designated as non-pathogenic variants including that in SCAC. Loss of heterozygosity (LOH) of chromosome 3p (which contains the VHL gene) was previously reported in SCA [1, 8]; however, none of our SCA or SCAC samples showed LOH in chromosome 3. A Clinvar pathogenic mutation in IDH1 (R132H) was seen in SCAC only, while no other pathogenic IDH1 mutations were found by Clinvar or FATHMM in the other specimens. IDH1 is an oncogene encoding isocitrate dehydrogenase 1, a component of NADPH metabolism, with mutations reported in glioma, cholangiocarcinoma and acute myeloid leukemia [12]. Germline WES was also performed, focusing on VHL and IDH1. One SCA patient had a Clinvar pathogenic germline VHL mutation that has been observed in VHL syndrome [13], although this patient did not exhibit clinical features of VHL syndrome. There were no germline pathogenic IDH1 mutations. In summary, only 3 of 5 (60%) of SCA exhibited either somatic or germline pathogenic VHL mutations, while SCAC had none, and a pathogenic somatic IDH1 mutation was exclusive to SCAC.

DISCUSSION

Definitive diagnosis of SCAC is difficult, as it was in this case. Diagnosis of SCAC was confirmed only after pathology confirmed similar histologic features from the primary tumor and the distant peritoneal metastasis. This diagnostic dilemma is seen across the literature. Imaging findings (CT or MR) or pathological results are unlikely to distinguish primary malignant SCN from benign SCN given their similar pathological and radiological presentations unless clear invasion or metastasis is seen [4–6]. About 25% of reported SCACs in the literature were initially diagnosed as benign SCN by surgical pathology and were subsequently designated as cystadenocarcinoma only when metachronous metastases were identified, suggesting the possible need for post-resection surveillance of some SCN [6]. A systematic review noted that the median time interval from diagnosis of SCN to metachronous metastases was 3 years (range 1–10) with a median primary tumor size of 10.5 cm (range 5–14) for those who developed metastases [4]. Thus, it is reasonable to offer post-resection surveillance for large SCN. Since the minimum size of SCAC reported in literature was 4 cm [4], for patients not being offered surgery for presumed benign SCA, ongoing surveillance could be considered for SCN with a size of at least 4 cm based on limited retrospective evidence. For patients undergoing non-operative surveillance, surgery is recommended if symptoms appear or if there is concerns for malignant conversion [7].

Surgical resection for patients with malignant SCN should be considered in patients with good functional status and operable disease status. For patients with inoperable disease, evidence for chemotherapy or radiation is limited. Of the 35 cases reported in literature, one patient with inoperable, stage IV serous cystadenocarcinoma was reported to receive a 4-month course of neoadjuvant chemotherapy with no response, although the patient had minimal progression during this time demonstrating the relatively indolent biology [3, 5]. Chemotherapy in combination with radiation therapy reduced tumor size and allowed for surgery in two cases [14]. Likewise, it is also unknown whether adjuvant chemotherapy is beneficial after resection of SCAC. In our case, we recommended close surveillance, and the patient remains disease-free after 32 months.

Consistent with the current literature, our 6 SCNs did not demonstrate mutations in KRAS, GNAS, RNF43 or CTNNB1 as described in non-serous cystic pancreatic lesions [1, 8]. Pathogenic somatic or germline VHL mutations were found in 3 of 5 SCAs (60%) but not in SCAC. Presence of VHL mutations may be helpful but not required for diagnosis of SCA, since VHL mutations are seen in only approximately half of the confirmed SCAs [1, 8]. None of our SCAC or SCA samples showed evidence of LOH in chromosome 3, as previously reported in some but not all SCA [1, 8]. Thus, neither VHL mutation nor LOH of chromosome 3 (which would alter VHL gene function) was necessary for SCAC development in our patient. The missense mutation in IDH1 (R132H) is noted in OncoKB for its oncogene status [12] and may serve as a genomic marker for SCAC. The IDH1-inhibitor ivosidenib has been FDA-approved for treatment of acute myelogenous leukemia and advanced cholangiocarcinoma [15]. This drug might have the potential to treat IDH1-mutant inoperable or recurrent SCAC, and thus, further study of this gene as a progression factor in SCAC is warranted.

In conclusion, we report a rare case of serous cystadenocarcinoma. Clinically, radiologically and pathologically distinguishing SCAC from SCA is difficult, with definitive diagnosis requiring synchronous or metachronous metastasis or organ invasion. Pathogenic VHL mutations were seen in some of SCA, but not SCAC. Further study is needed to determine whether this gene (or alterations thereof via LOH) plays a role in pathogenesis of SCN. A targetable pathogenic somatic mutation in the oncogene IDH1 was unique to SCAC and warrants further study as a progression factor.

ACKNOWLEDGEMENTS

We appreciate the assistance of Drs Ralph Hruban and Elizabeth Thompson of Johns Hopkins Department of Pathology for their pathologic review of this case. We also would like to acknowledge the Markey Cancer Center Biostatistics and Bioinformatics Shared Resource Facility for statistical analyses. Molecular analysis (Germline and Somatic WES) was performed through the Oncology Research Information Exchange Network (ORIEN) Total Cancer Care protocol, a multi-institutional oncology genomics protocol established at Moffitt Cancer center in collaboration with M2GEN (Tampa, FL).

CONFLICT OF INTEREST STATEMENT

M2Gen Travel Award for Travel to ORIEN Annual Scientific Retreat to present this study (M.J.C.).

FUNDING

Markey Cancer Center Support Grant NCI P30 CA177558 (C.W.).

References